p38 MAPK
p38 Mitogen
Activated Protein Kinase
BLAST
BLAST stands for the Basic Local Alignment Search Tool in bioinformatics. It is a heuristic alignment algorithm to compare amino acid or nucleotide sequences to a sequence database. This enables similarity regions between the sequences to be found, and calculatation of the statistical significance of the alignments.
An NCBI protein-protein BLAST (blastp) was used to compare the sequences of p38MAPK paralogues MAPK alpha, beta, gamma and delta against the NCBI non-redundant protein sequence (nr; all non-redundant GenBank CDS translations, PDB, SwissProt, PIR and PRF excluding environmental samples from WGS projects; 2016/03/19) and Refseq (refseq_protein; NCBI Protein Reference Sequences; 2016/03/19) databases. Query sequences were representative orthologues from 6 species: Homo sapiens (human), Macaca fascicularis (crab-eating macaque), Mus musculus (house mouse), Rattus norvegicus (Norwegian rat), Danio rerio (zebrafish), and Xenopus tropicalis (Western clawed frog).
On the accompanying webpages, the graphical images show the results of searches against the nr database, whilst the detailed results show the output from searches against the Refseq database.
Max Score: The score of best aligned sequence.
Score: a score of the alignment which allows to compare with other proteins.
Total Score: The sum of all aligned sequence scores.
Query Coverage: The precentage of alignment of the query sequence.
Expect (E) value: a parameter which describes the number of alignments you expected by chance, with the observed or higher scores. Lower the E values, more significant of the alignments (usually E < 0.05), despite shorter alignments have a relatively higher E values.
The results of p38 MAPK paralogues and orthologues are presented below.
Comparing the human p38 paralogues, p38α is 73%, 64% and 61% identical to p38β, γ and δ.
Comparing the zebrafish p38 paralogues, p38α is 73%, 61% and 62% identical to p38β, γ and δ.
Comparing the macaque p38 paralogues, p38α is 76%, 64% and 63% identical to p38β, γ and δ.
Comparing the mouse p38 paralogues, p38α is 76%, 63% and 64% identical to p38β, γ and δ.
Comparing the rat p38 paralogues, p38α is 74%, 63% and 62% identical to p38β, γ and δ.
Comparing the frog p38 paralogues, p38α is 81%, 68% and 65% identical to p38β, γ and δ.